Plasmid DNA Extraction (Midiprep)
Plasmid DNA Extraction (Midiprep)
15.5K viewsMay 18, 2010
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Tomasz Grzywa on Instagram: "Plasmid amplification & midiprep day 🧬 Midiprep is a standard method to amplify and purify plasmid DNA from bacteria. It starts with bacterial transformation, where plasmids carrying a gene of interest are introduced into E. coli. Transformed bacteria are then plated on agar plates (first video), and a single colony is picked and inoculated into a larger liquid culture to clonally amplify bacteria carrying the plasmid. After bacterial growth, cells are harvested, ly
Tomasz Grzywa on Instagram: "Plasmid amplification & midipre…
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